FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48118537
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC97300.019.0312044
GACCGCG98050.018.816795
CAGACCG101300.018.5684683
ACCGCGT99650.018.4679346
CCGTACG53800.017.397644
TTCGGCG52250.017.13768444
CGTACGC54600.017.0191045
CCGCGTT108200.016.9879577
CGCGTTC112100.016.4770648
AACGCTA106400.015.49909823
AAAACGC99650.015.48784221
ACGCTAC108450.015.30984924
GCCGGCT134800.015.2780011
TCGGCGC59400.015.26419645
GTCCGTT37150.014.7993011
CGCTACC113500.014.50980425
CCCGTAT41300.014.403371
GAAAACG107650.014.39953220
TCACGCT151200.014.343918
ACGCTCA157800.014.17169110
Produced by FastQC (version 0.11.5)