FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48118537
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG110850.012.33584438
AGCTTAG119050.011.58055545
GGCATAG114800.011.53902235
GGTCACT187350.010.4277977
CATAGGG131150.010.25523637
GGGAGGT216600.010.0973933
ACCCGGC66500.09.980915
GTCACTC200200.09.9611618
GAGCTTA140700.09.6711444
GCATAGG140000.09.51056936
TGGCATA142850.09.44640534
GGAGGTC220500.09.418654
GAGGTCA231100.09.3268575
ACTCTCC214400.09.31220811
GTTCTAT133250.09.0520341
TAGGGGA221150.08.8273139
TCACCCG83900.08.7161113
CACTCTC232400.08.55214710
GCACCGA195550.08.44218144
CGTAGGG70350.08.44019737
Produced by FastQC (version 0.11.5)