FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46945996
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1611750.34332001391556377No Hit
CCCCACGATGCTGTCCAGCTGCCTCCTGAGGTTGTTGATGTACTGCTCGAA523360.11148128585875566No Hit
CTCATACTTGTTCTTGAAGTCTTCCACCAGGTCCTGCATGTTTCTCAGCTC478300.10188302320819863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC365450.025.801614
CAGACCG368150.025.655163
GAACGAT327400.024.47017345
GACCGCG385250.024.4693555
ACCGCGT394900.023.842926
CCGCGTT402100.023.4159897
CGCGTTC402850.023.3775568
TCAGACC421350.022.639382
CTCAGAC453900.021.9106941
AGAACGA369950.021.67396744
GCGTTCT441800.021.3929279
CGTTCTC480850.019.898910
AATACGG407000.019.67462332
TACGGAG409750.019.52496334
CCCAATA416250.019.3943329
ATACGGA413450.019.37766633
AAGAACG414550.019.36382743
CAATACG431900.018.7070231
ACGGAGA435350.018.39740635
CCCCAAT442950.018.3270128
Produced by FastQC (version 0.11.5)