FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45446294
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG103050.010.938807545
ATAGGGG105300.010.87599938
GGCATAG103400.010.81472935
GGGAGGT170600.010.3799563
CATAGGG123150.09.26303637
GGAGGTC185850.09.1286844
GCATAGG125000.09.12594236
ATAACGC54250.09.04141739
TGGCATA127400.08.97168634
TCGCGCT25700.08.84236745
TCAGACG64150.08.7337862
GACGCAA40450.08.62170144
GAGCTTA133700.08.56576544
TAACGCC57350.08.55269240
GTTGTCG17050.08.44569526
CGTGGCG75150.08.4135091
TAGGCTA59950.08.40696210
CGTCAGT80800.08.2985991
TTACGCG8250.08.18176735
ACTCTCC203000.08.09109111
Produced by FastQC (version 0.11.5)