FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47828440
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT651540.1362243886691684No Hit
CTCGTACTGAGACTCAAGGCCGTCTCCACAACTCCAACCAGTGCAAATGAC539020.1126986370452392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT151150.020.85930845
AGACCGC155350.020.6192254
CAGACCG164250.019.6526683
GACCGCG169450.018.8636685
ACCGCGT174000.018.4079276
CCGCGTT173950.018.4002887
CGCGTTC179100.017.8837478
AGAACGA179650.017.5752444
CCGTACG54950.017.4796924
CGTACGC54550.017.1955055
CCCGTAT29800.016.088041
AATACGG195650.016.07531532
ATACGGA197850.015.88594233
TACGGAG196950.015.83362334
TCAGACC216350.015.4399672
TACGCCA61700.015.4205317
AAGAACG205500.015.35660343
TTCGGCG61900.015.18223544
GCGTTCT213500.015.0339139
CAATACG212100.014.97637531
Produced by FastQC (version 0.11.5)