FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47828440
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG111550.011.84867538
AGCTTAG113100.011.78165945
GGCATAG115000.011.55168935
CATAGGG127850.010.47148837
GCATAGG132200.010.03687936
GGGAGGT203900.09.8385023
GAGCTTA137900.09.76342244
TGGCATA140300.09.49410534
ACTCTCC198450.09.354218511
GTCACTC200750.09.123718
GGAGGTC218500.08.8620174
TAATCGG35550.08.5403384
TAGGGGA221450.08.07910239
ACCCGGC62850.08.0140595
ACGCGCG13350.07.743184620
CACTCTC236400.07.690572310
CGTAGGG61850.07.665385737
CGTGGCG72200.07.63189741
GTAACGA32700.07.62893318
ACACTCG67700.07.5740314
Produced by FastQC (version 0.11.5)