FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45372177
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG575410.12682001130340295No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA482740.10639560010532445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT299500.014.14699653
AGCTTAG211250.013.30185745
GGAGGTC318800.013.1914734
GGTCACT316950.013.0113137
GTCACTC322550.012.65986258
ATAGGGG227550.012.398828538
GGCATAG229500.012.17637535
GAGGTCA377050.011.5528855
GAGCTTA245550.011.39803144
TCACTCT365400.011.3722739
ACTCTCC361750.011.36263111
TGGGAGG268150.011.3095832
TGGCATA252250.011.11394834
GTTGTCG20100.010.96884226
ATCCTAG103300.010.4325236
AGGGATC145300.010.3351552
TATCCTA100650.010.1095795
TCCTAGG103750.010.0835697
CATAGGG286450.010.01437137
TAGGCTA96400.09.98885310
Produced by FastQC (version 0.11.5)