FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45358901
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG56950.08.37799238
GTTGTCG22000.08.28252726
TCAGACG69750.07.7409522
GTAGGGT30350.07.6343816
TAATCGG28650.07.6175974
TAGGGTA29650.07.511138417
GGCATAG63950.07.49485335
CGACCCG91000.07.2447756
GCATAGG63350.07.210904636
CATAGGG64650.07.2055837
CGTAACG17500.07.20301642
TTAACGG35200.07.0305273
TGGCGAC95700.06.9820413
TCTATAG240650.06.9647943
CAGACGT71400.06.963573
AGCTTAG70850.06.957754645
CGTGGCG94450.06.8836521
TTCTATA261050.06.8167932
ATTTCTA246700.06.8120652
GCGACCC98800.06.80932145
Produced by FastQC (version 0.11.5)