FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46941889
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT815810.17379147226052194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT193400.020.917245
AGACCGC215950.018.7536544
CAGACCG224850.018.1314243
GACCGCG225900.017.867875
ACCGCGT227250.017.8296626
AGAACGA237250.017.0429644
CCGCGTT238800.016.9767027
CGCGTTC241450.016.7996398
GTCCGTT36150.016.5663851
CGTTACG12100.015.80493621
AAGAACG269300.015.08141843
TCAGACC274400.015.0459142
AATACGG272700.014.88652932
TACGGAG275200.014.73534934
GCGTTCT277650.014.7065059
ATACGGA277900.014.63267333
CAATACG285250.014.29342131
CTCAGAC328050.013.8975881
CGTACGC62050.013.5974065
AACGCTA112500.013.55938723
Produced by FastQC (version 0.11.5)