FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49003945
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCAC824720.16829665448363393No Hit
CCACGGTGAGCTTGCTGTAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG559380.11414999343420208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG19950.019.5066156
GTCCGTT37450.017.906211
CGTTACG22500.017.69580721
CCGGCTT188200.017.2235832
TCACGCT175600.017.024698
TTCACGC177500.017.0198757
AACGCTA172250.016.7421623
ACGCTAC174600.016.52970524
ACGCTCA191800.015.9854910
CGCTACC182550.015.79751925
CCCCCCT37750.015.4154591
AAAACGC188800.015.35785421
GCCGGCT173750.015.3071571
CGCGTGT14150.015.100971
GAAAACG194200.014.8844620
CGGCTTC230750.014.6616883
CCCCCGT32350.014.6157971
TCGACGA27550.014.4520817
CACGCTC210850.014.3811969
AAACGCT204450.014.24837622
Produced by FastQC (version 0.11.5)