FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42174440
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG850950.201769128410478No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA428540.10161130770201099No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG424720.1007055458234893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT374800.016.8815253
GGAGGTC382300.016.3324974
GAGGTCA426450.014.9476695
AGCTTAG296950.014.49419245
TGGGAGG309450.014.4096752
GGTCACT430050.014.3740037
ATAGGGG301950.014.23410838
GGCATAG302200.014.20600635
GTCACTC440650.014.0746598
CTGGGAG344350.014.014851
GTTGTCG25900.013.55316526
TCACTCT460700.013.5158639
GAGCTTA321750.013.43294344
TGGCATA321200.013.33692834
ACTCTCC497750.012.50570711
AGGTCAC523000.012.15084656
CACTCTC508300.012.09084110
AGGGATC151450.011.8751122
AGGCTCT389950.011.30714220
ACCCGGG88250.011.0441772
Produced by FastQC (version 0.11.5)