FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58591479
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG855340.14598368476071408No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA636940.10870864003962079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT429200.014.7038063
GTTGTCG43550.014.15292926
GGAGGTC451700.013.8720484
GAGGTCA478950.013.2199175
GGTCACT465450.013.1255187
AGCTTAG322000.013.12066845
GTCACTC495150.012.4700148
ATAGGGG339200.012.24835738
GGCATAG351100.011.90860435
TGGGAGG385050.011.5269382
ACTCTCC544950.011.45010611
TCACTCT554700.011.407149
GAGCTTA372700.011.20294344
TGGCATA377850.011.08865334
CACTCTC563250.011.06610710
ATATAGC65550.010.84760956
ACCCGGG125250.010.1299052
CTGGGAG488100.010.0427361
GCTAAGT63200.010.00330814
TGTCGTT66400.09.82489628
Produced by FastQC (version 0.11.5)