FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54818385
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA971290.17718325704049837No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG929860.16962557361002153No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG818500.14931122104381586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT455300.014.1928253
GGAGGTC460850.013.6443614
GAGGTCA498150.012.8265885
GGTCACT503850.012.1763567
AGCTTAG345500.012.00543745
GTCACTC523800.011.6772918
ACTCTCC536500.011.41747311
TCACTCT546400.011.3622349
GGCATAG361100.011.36078135
ATAGGGG366450.011.36050638
TGGGAGG379000.011.3548142
GTTGTCG26150.011.26893326
CACCCGG138150.011.2586711
CACTCTC545650.011.1828710
ACCCGGG133450.011.0978442
CTGGGAG430300.010.791651
GAGCTTA386050.010.73476144
AGGTCAC591200.010.7061996
TGGCATA392200.010.55092534
CGTGGCG199100.010.4010341
Produced by FastQC (version 0.11.5)