FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47571452
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2244430.47180186974322325No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA600340.12619753544625884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC441050.024.3834724
GAACGAT445800.023.94686545
CAGACCG452600.023.8258363
GACCGCG456800.023.5132125
ACCGCGT457800.023.4950856
CCGCGTT470950.022.8339587
CGCGTTC471800.022.7880548
TCAGACC496200.021.8371492
GCGTTCT505700.021.2826779
CTCAGAC527500.021.2477781
AGAACGA520900.020.51128444
CGTTCTC566100.019.19469310
CCTCTCA580200.018.75925819
AAGAACG578600.018.45307543
AATACGG583650.018.34255632
TACGGAG585400.018.21134434
ATACGGA586200.018.20928833
ACTCCCC595000.018.15670425
CAATACG602900.017.79374131
ACGGAGA605600.017.64882935
Produced by FastQC (version 0.11.5)