FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47571452
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA709930.14923446103768284No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG565470.11886750902621178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAT255200.014.5397741
AGCTTAG196600.014.0061445
GGGAGGT295850.014.0052563
GGCATAG204950.013.44598935
ATAGGGG211250.013.12576438
GGAGGTC318000.013.0790544
GAGCTTA224300.012.34367944
ACTCTCC329900.012.2711811
TGGCATA228000.012.09203834
TCTATAG364900.012.0019653
TTCTATA378350.011.7654542
CACTCTC365600.011.1711510
CTATAGT393250.011.1308674
AGGGATC140200.011.048632
GGTCACT364600.011.0326267
CATAGGG252700.011.0079737
ATAGTGT403900.010.9473916
TATAGTG402250.010.901875
TGGGAGG277300.010.6532652
GTCACTC384450.010.6039778
Produced by FastQC (version 0.11.5)