FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41800106
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCG427970.1023849078277457No Hit
CTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCC418840.10020070284032295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGGA215350.015.3019241
TGTGCAA194100.013.31723712
GGTTCTT197700.013.04238136
GACCTCC255550.011.9950995
GTGCAAG236250.011.29812913
TATCCTA66800.011.202975
ATAGGGG58700.011.17631638
AAACACC203250.010.97992426
CGTCAGT119750.010.9621111
TAGGCTA62950.010.88918810
ATGAAAC248650.010.62271323
AGCTTAG60800.010.62220545
GTGGTTC247200.010.58333634
ACCTCCT285550.010.5041776
TGTGGTT258700.010.26625433
GTTCTTC261100.010.19590837
ATCCTAG83650.010.1797286
ATAACGC98400.010.17943539
CCTAGGC70200.010.0514638
GGCATAG63600.010.03599735
Produced by FastQC (version 0.11.5)