FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54781555
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3556620.6492367732168245No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1582000.28878333227306163No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1214110.22162751678005488No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG1066240.19463485474262276No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC674610.12314546383358413No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA624910.11407306711173132No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC595700.1087409804267148No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG577760.10546615553355505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG979100.018.588413
AGACCGC976350.018.5531794
GACCGCG985000.018.3468175
TCAGACC1012200.018.1385042
ACCGCGT1006850.017.984426
CTCAGAC1061650.017.6275521
CCGCGTT1108550.016.3340267
CGCGTTC1114400.016.1675998
CGCGTAT6700.015.4504781
GCGTTCT1180850.015.3264889
CGTTCTC1229650.014.85579310
CCCGCGT33850.014.7588731
CGTTACG27350.014.72504621
GAACGAT1213500.014.70141245
CTACACG151600.014.54478545
ACTCAGA596150.013.6084481
CGACCGC17200.013.6070441
CCCCGCT86850.013.2665821
GTTCTCT1391350.013.19396611
CCGTACG73050.013.12089354
Produced by FastQC (version 0.11.5)