FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48271110
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1910280.39573981207392994No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG525220.10880628185264435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT322650.028.42112545
AGAACGA378950.024.27011344
AGACCGC385850.023.8009174
CAGACCG392200.023.4497623
ACCGCGT404100.022.7482876
GACCGCG414350.022.1421155
CCGCGTT416200.022.1139647
CGCGTTC425750.021.6390638
TCAGACC438550.021.0944962
AAGAACG439650.020.93462643
AATACGG439750.020.87037532
ATACGGA441900.020.75351533
TACGGAG442250.020.69095834
CAATACG456200.020.2016431
CTCAGAC486100.020.0507281
GCGTTCT462050.019.9877269
ACGGAGA460600.019.8422235
CGGAGAG472600.019.39546836
CCCAATA493000.018.74414329
CCCCAAT512200.018.08106628
Produced by FastQC (version 0.11.5)