FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48271110
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG781570.16191258083768947No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG596940.1236640300999915No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA558620.11572553438278091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG248400.015.07917245
GGGAGGT368450.015.0572753
GGCATAG259400.014.33094935
ATAGGGG261600.014.29850838
GGAGGTC389600.014.0844544
GAGCTTA279400.013.462502544
TGGCATA278200.013.35399934
ACTCTCC409500.013.33533511
GGTCACT416150.012.992457
GTCACTC435600.012.5414838
TGGGAGG315700.012.4137032
GTTGTCG22700.012.0899626
CATAGGG312300.012.00508837
CACTCTC459050.011.94983210
GCTCTGT470600.011.54371922
TCTCCCC475150.011.46900213
TGTCCAA479700.011.32500326
GCATAGG332300.011.30255536
CTGGGAG390800.011.20241
TCAGACG85300.011.1826472
Produced by FastQC (version 0.11.5)