FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42611117
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1008110.2365838004199702No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG517400.12142371203270734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC205750.023.3626144
CAGACCG209600.023.0408213
GACCGCG225650.021.390955
ACCGCGT236450.020.4231576
TCAGACC267850.018.4585862
CCGCGTT263650.018.340977
CTCAGAC298300.018.1521841
CGCGTTC268850.017.9693588
TTCGACG13400.017.4646976
GCGTTCT294100.016.4551339
CGTTACG28200.016.11605821
CCGTACG98300.015.0866184
CTACACG42300.014.73433845
CGTACGC101450.014.5730825
CGTTCTC352050.014.11045710
TCCGTAC107650.013.9852933
TACGCCA112100.013.3484137
TCGTTAC20750.013.33763244
GTACGCC115050.013.1631836
CCCGTAT26550.013.05941
Produced by FastQC (version 0.11.5)