FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51260398
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT858030.16738652711982455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC190800.023.5835254
CAGACCG194950.023.162233
GACCGCG205500.021.699465
ACCGCGT213500.020.9917476
CCGCGTT224450.019.937577
CGCGTTC227800.019.6937548
CTCAGAC288500.018.0579531
GCGTTCT248700.018.0297059
TCAGACC263700.017.8995232
GAACGAT284550.015.61676245
CCCGTAT30600.015.537731
CCCGGAT116250.014.8865751
CGTTCTC320650.014.48189410
CGTTACG20750.014.20634421
CCGGCTT216600.014.1989472
TTCGACG14800.014.1376856
AATACGG318750.013.91892732
ATACGGA320100.013.8813133
TACGGAG315850.013.84015434
AGAACGA322450.013.83586844
Produced by FastQC (version 0.11.5)