FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51260398
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT220800.011.1888183
ATAGGGG152850.011.0811138
GTTGTCG25400.010.71356926
GGCATAG160600.010.42053435
GGAGGTC232750.010.3904824
AGCTTAG163900.010.23378845
CGTAACG10500.09.84995142
GGTCACT238600.09.5879027
GAGCTTA181000.09.32914344
TGGCATA184150.09.19847834
GTAACGA60150.09.01648618
GTCACTC255000.09.0066158
AGGGATC142050.08.9405462
ACTCTCC260200.08.935266511
TAATCGG63600.08.8866424
CATAGGG193850.08.77248937
ATAATCG67000.08.6720463
AATCGGT69700.08.593175
GCATAGG200450.08.472424536
ATATAGC50950.08.3524546
Produced by FastQC (version 0.11.5)