FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46388349
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG496030.10692986723886207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG36550.026.2215166
CGACGAG44250.021.4044638
CGGTATA57950.021.1617320
TCGACGA48200.020.1171197
ACCCGTC35150.018.88414432
GTTCGAC52200.018.0583925
TCGTATG37600.017.53136845
CGTTACG18600.016.69739321
TTCGGCG118750.016.04670344
GTCCGTT33100.015.9116061
GTATAGA86200.015.6912422
AGTTCGA64200.015.3137644
ATAGAGT88300.015.16505424
TCGGCGC128500.014.88167645
AGACCGC117850.014.5656714
GACGAGG84950.014.2745019
GAACGAT121000.014.11212545
CGAGGAT76900.014.07195111
GCCGGCT196700.014.017091
ATGACGG84100.013.96444416
Produced by FastQC (version 0.11.5)