FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46388349
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT38900.016.7473811
TCGTGTA39800.016.31222714
GTCGTGT42050.015.27912613
TGTAGGG49300.013.85240417
GTAGGGA50900.013.46109918
AGTGTAG51000.013.39065628
GTCGCCG61550.013.35832444
TCAGACG117500.012.0639632
GTAGATC58200.011.84987731
CGTCGTG56400.011.59077212
ACCCGGC71650.011.5693425
AGCGTCG58650.011.56744410
GGTCGCC58150.011.51296843
ATCTCGG64200.011.47723635
TAGCGTA8950.011.41048237
AACTCTA87700.011.19388445
GGGAGGT233050.010.7189973
GTTGTCG19100.010.58540826
TAGATCT65850.010.47324132
GTCACTC221400.010.4611528
Produced by FastQC (version 0.11.5)