FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45248935
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG705020.15580919197324755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG246650.014.78373545
GGGAGGT371750.014.7300633
GTTGTCG24250.014.19662926
GGAGGTC380500.014.139214
GGTCACT378550.013.833357
ATAGGGG267600.013.61537938
GGCATAG266600.013.54857235
GAGCTTA273150.013.30822644
GAGGTCA413450.013.2685445
GTCACTC399750.013.0491958
TGGCATA280900.012.89070634
ACTCTCC424900.012.288959511
TCACTCT437950.012.1223769
TGGGAGG323000.011.9260492
CACTCTC446450.011.68043910
CTGGGAG376450.011.1612211
CATAGGG334650.010.995237
ATATAGC38900.010.9864836
ACCCGGG110500.010.8876272
CACCCGG118250.010.5758631
Produced by FastQC (version 0.11.5)