FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46615563
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1950620.41844823369397044No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG498220.1068784688924598No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA477600.10245505347645377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT333250.026.61723145
AGACCGC380150.023.3154834
AGAACGA386800.022.96000744
CAGACCG388600.022.889543
GACCGCG431650.020.5335465
GCGTTCT435900.020.384329
ACCGCGT440550.020.1996926
CCGCGTT451750.019.7189147
CTCAGAC475300.019.6009621
AAGAACG453550.019.5561343
CGCGTTC456400.019.5327958
TCAGACC463750.019.4277612
ATACGGA456700.019.34330733
TACGGAG457950.019.3292534
AATACGG461150.019.22690432
CAATACG479000.018.56420931
ACGGAGA481150.018.42526635
CCCAATA489650.018.1687729
CGTTCTC502600.017.90738110
CGGAGAG495700.017.82536936
Produced by FastQC (version 0.11.5)