FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47535845
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA707830.14890447408687066No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT511750.10765560178850296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG108450.025.3093535
ACCGCGT110500.024.7371526
AGACCGC111300.024.6012214
CCGCGTT123000.022.2046187
CAGACCG126250.021.8840773
CGCGTTC126600.021.5376668
TTCGACG22400.020.6897346
ACCCGTC38250.019.7635532
CGTCCCG44500.017.64490735
TCGTTAC26800.017.5444944
TCAGACC165800.017.2210772
TCGTATG44950.017.21702245
GCGTTCT163000.016.7694329
CTCAGAC210450.016.160241
CGGTATA37450.016.10164620
TCGACGA28400.016.0807917
CGTTACG30250.015.91806621
CTCCGAT40600.015.5534741
CCGTACG17100.015.5255574
GTTCGAC29250.015.2296555
Produced by FastQC (version 0.11.5)