FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47535845
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT37450.019.28311
GGTCGCC41200.018.23960743
TCGTGTA43750.016.50654414
GTCGCCG47450.015.64728344
AGCGTCG48750.015.22809810
CGTCGTG48250.015.19999912
GTCGTGT48800.014.93669113
TGGTCGC57950.014.67818142
TCTCGGT57350.013.30113636
ATCTCGG67000.012.39197835
CTCGGTG67250.011.94552437
GTAGGGA64800.011.87281818
GTTGTCG25450.011.84746126
GGAGGTC170450.011.7788454
GTAGATC68500.011.72634331
AGAGCGT66600.011.7208318
CGTAACG17850.011.47194542
TGTAGGG70250.010.9201517
GGGAGGT196050.010.7574073
ATAGGGG143550.010.722157538
Produced by FastQC (version 0.11.5)