FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51061446
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT948120.18568216810781268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC223450.020.8323294
GAACGAT226150.020.41974645
CAGACCG234200.020.0009923
ACCGCGT233800.019.938986
GACCGCG236500.019.6732945
CCGCGTT243650.019.252847
CGCGTTC249750.018.78268
AGAACGA263700.017.59738344
TCAGACC280800.017.0983982
TTCGGCG69600.016.7813844
GCGTTCT291750.016.1943519
CCGTACG72300.016.1816484
CGTACGC73300.016.052975
TCGGCGC74500.015.91929745
AATACGG290850.015.87362932
CTCAGAC339050.015.7705291
ATACGGA293600.015.68663233
TACGGAG295300.015.53511534
AAGAACG300750.015.42948143
CAATACG307700.015.1067431
Produced by FastQC (version 0.11.5)