FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52499407
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2363730.45023937127518415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT375600.030.0471145
AGACCGC441900.025.6458074
AGAACGA441400.025.63423244
CAGACCG447700.025.3991893
ACCGCGT468600.024.1697586
CCGCGTT475850.023.7873257
CGCGTTC479450.023.650958
GACCGCG481100.023.5137185
TCAGACC493850.023.2582682
AAGAACG493100.022.96481743
GCGTTCT509150.022.6601969
ATACGGA504300.022.36506333
TACGGAG504400.022.33386634
AATACGG508000.022.19773932
CTCAGAC566250.021.5975671
CAATACG533100.021.21590631
ACGGAGA533200.021.16979235
CCCAATA546600.020.8030529
CGGAGAG539950.020.75604636
CCCCAAT560250.020.29218528
Produced by FastQC (version 0.11.5)