FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52499407
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG162550.014.30915645
ATAGGGG163950.014.13583338
GGCATAG163900.014.04191735
GAGCTTA188600.012.4182644
GGGAGGT269200.012.3225353
CATAGGG192200.012.10422737
TGGCATA193100.012.09197434
GTTGTCG23250.011.90057726
GCATAGG204400.011.41394836
GGAGGTC293350.011.2466574
AGCGCTC65450.09.8338159
ACTCTCC337500.09.79509511
TCAGACG77050.09.6692292
TGTCCAA351300.09.31048126
CACTCTC361050.09.26215710
TGGGAGG279100.09.2580292
TAGGGGA341450.09.24542739
TCTCCCC362950.09.10802113
AAGCCGG224050.09.08623845
GCTCTGT354750.09.06854422
Produced by FastQC (version 0.11.5)