FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57452050
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT851820.14826624985531414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT195150.021.78708845
AGACCGC226750.018.7109974
CAGACCG236250.018.072863
AGAACGA242000.017.5876744
CGTTACG18000.016.88786921
CCGTACG131100.016.5758934
GACCGCG258750.016.2578745
CGTACGC134450.016.129425
TTCGGCG136950.016.11422544
ACCGCGT266100.015.8510816
CGCGTTC274150.015.4020518
CCGCGTT274450.015.3770177
ATACGGA279300.015.37631433
AATACGG279450.015.32783532
TCCGTAC145300.015.1881863
TACGGAG281850.015.17334534
TACGCCA145050.015.1368187
TTCGCGC21050.014.9616157
GTACGCC150350.014.7528526
GTCCGTT43950.014.7490511
Produced by FastQC (version 0.11.5)