FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57452050
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG191300.014.813889545
GGCATAG204450.013.71432235
ATAGGGG212550.013.30997838
GAGCTTA218450.012.99335744
GGTCACT318650.012.6682877
GGGAGGT350400.012.6104133
GTCACTC327400.012.5015588
CATAGGG232750.012.105865537
TGGCATA232850.012.07963334
ACTCTCC344750.011.82011311
GCATAGG247600.011.388436
GGAGGTC377000.011.1240494
TAGCGTA11050.010.99797437
ATAGCTC87200.010.99502639
ACCCGGC95650.010.6090285
TCACTCT401900.010.5704669
CACTCTC395400.010.32874210
AGCATAG120200.010.28078445
TCAGACG114500.09.8824642
TGGGAGG316950.09.79472
Produced by FastQC (version 0.11.5)