FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53685555
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG807660.15044270288348513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG255400.014.94967445
GGGAGGT426850.013.7257373
GGAGGTC415000.013.6026314
ATAGGGG282200.013.49807738
GGCATAG281000.013.44362435
GAGCTTA284650.013.32649444
TGGCATA302900.012.56077234
CCGTACG20300.011.8592734
CATAGGG333450.011.50444437
TGGGAGG359150.011.1948482
ACTCTCC498950.011.16567611
GCATAGG356550.010.77802936
TAGGGGA504100.010.76099739
CGTACGC23500.010.5316595
CTGGGAG427900.010.3620871
AGCATAG160900.010.33377845
CACTCTC543550.010.31986910
GCTCTGT540700.010.26139222
TGTCCAA548950.010.16458526
CACCCGG134600.010.1551761
Produced by FastQC (version 0.11.5)