FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47191799
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG144200.012.4202438
AGCTTAG144650.012.30389145
GTTGTCG25600.011.4257626
GGCATAG153650.011.42205435
GGGAGGT231200.011.2110513
GAGCTTA164050.010.72543744
GGTCACT226150.010.72516257
GTCACTC227600.010.6766058
GGAGGTC248250.010.3866884
CATAGGG174550.010.1962137
TGGCATA173350.010.18890634
ACTCTCC242450.010.1433111
AGGTCAC258850.09.9178886
GCATAGG183950.09.66294436
GAGGTCA279100.09.3998395
TCACTCT276800.09.0552589
CACTCTC273800.08.71072210
TAGGCTA76850.08.66621710
TGTCGTT37750.08.52316328
TTAACGG62150.08.5076273
Produced by FastQC (version 0.11.5)