FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47961033
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG857340.17875761766849352No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT673350.1403952245982692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC156400.022.339774
GCCGGCT222250.022.0975171
CAGACCG162350.021.6596133
GACCGCG166550.020.9918355
TTCGACG22250.020.9307296
CCGGCTT278000.020.0309722
ACCGCGT175750.019.9313776
TCACGCT287300.019.1542368
TTCACGC289150.019.0550297
CCGCGTT189200.018.5263717
AACGCTA286950.018.51485323
ACGCTAC287800.018.48362724
CGCGTTC193450.018.1542438
CGCTACC297350.017.85971625
AAAACGC306250.017.37008321
ACGCTCA321350.017.2716910
GAAAACG311250.017.09761420
TCGACGA27250.017.0902297
CGGCTTC345350.016.9182913
GTTCGAC28900.016.737275
Produced by FastQC (version 0.11.5)