FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54338924
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG30450.013.67033526
ATAGGGG157000.013.24525238
AGCTTAG164750.012.49884845
GGGAGGT211300.012.317153
GGCATAG165100.012.25348835
GGAGGTC227750.011.5461424
GAGCTTA184400.011.2406744
CATAGGG184100.010.98907237
GGTAACG58350.010.79552817
GCATAGG189300.010.68686236
TGGCATA194100.010.56171334
ATAACGC65650.010.55868639
TAACGCC66650.010.43405340
GTAACGA58750.010.37768718
AATCGGT66450.010.2235075
TAATCGG64800.010.2059414
ATCGGTT70250.010.0867536
TGTCGTT44900.09.772943528
ATAATCG70750.09.5383043
ACTCTCC275250.09.39885311
Produced by FastQC (version 0.11.5)