FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47648331
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG106350.013.03615545
GGCATAG113850.012.33340835
ATAGGGG120700.011.87597238
CATAGGG126150.011.29141637
GAGCTTA128300.011.01615844
GCATAGG134350.010.61897736
TGGCATA138000.010.27283434
GGGAGGT218000.09.9172723
AGCGCTC67700.09.7694139
GCGTTCG19250.09.46660415
CGTGGCG109150.09.2753341
ATAGCTC61450.09.22817139
ACCCGGC67900.09.0450585
CGGGACG58350.09.02239317
CGTAGGG63250.08.85871637
CGCGTTC20900.08.82671814
GCGTAGG63900.08.73337136
CGTTCGC22150.08.63353716
TAGGGGA229800.08.61726939
CCGCGTT22200.08.6136413
Produced by FastQC (version 0.11.5)