FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53431649
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT618720.11579653848976287No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG573400.1073146741175815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT233750.022.27330845
AACGCTA179050.019.36261223
ACGCTAC181550.019.13403524
AGACCGC281750.018.5972044
CGCTACC187800.018.53428625
CAGACCG289450.018.1568373
AGAACGA294800.017.72999444
TCACGCT208400.017.4876148
AAAACGC202800.017.20532221
GCCGGCT195850.017.1774581
ATACGGG130300.016.96106333
GAAAACG191000.016.64288320
TACGGGG133550.016.54880534
AAACGCT212200.016.52816222
GACCGCG318050.016.4675755
TTCGGCG126300.016.42534344
TTCACGC225750.016.3728057
AATACGG318550.016.36803832
CCGGCTT239300.016.2270582
CAATACG332100.015.74052431
Produced by FastQC (version 0.11.5)