FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53431649
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG156650.012.39716145
GTTGTCG21050.012.18643426
ATAGGGG167100.011.34100438
GGCATAG166400.011.1848435
TCAGACG109950.011.1057342
CGTAACG12750.010.59117942
GGGAGGT266200.010.4582773
GAGCTTA186450.010.33150944
GGTCACT258300.010.1972387
GTCACTC267700.09.8736078
ACTCTCC268350.09.750669511
CCGTACG18950.09.7320394
GGAGGTC288700.09.6988844
TGGCATA197050.09.55916934
CGTGGCG197350.09.4693191
CATAGGG198750.09.46711937
AGGTCAC297550.09.4033026
GTGGCGA200350.09.1701282
GCATAGG206650.09.06074536
GAGGTCA322750.08.8640285
Produced by FastQC (version 0.11.5)