FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49186442
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCG152400.011.0629781
AGCTTAG131100.010.97016645
TCAGACG93150.010.7531782
ATAGGGG141750.010.41303738
GGCATAG139150.010.39619735
GGGAGGT221850.09.9228213
TGGCGAC179450.09.4576583
CGACCCG179850.09.4458616
GGAGGTC230650.09.4074564
GAGCTTA156800.09.3587544
GGCGACC180100.09.27339654
GCGACCC184800.09.267935
GTGGCGA181150.09.2198792
CATAGGG157150.09.17696837
GCATAGG159150.09.11804236
CAGACGT110350.09.0362263
TGGCATA167100.08.72431234
ACTCTCC244750.08.45797111
CGTAGGG70700.08.27388637
GCGAACG22100.08.24523919
Produced by FastQC (version 0.11.5)