FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45410147
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT656870.14465269183118917No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG539800.11887211023562641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG162750.021.2189715
ACCGCGT164250.021.0250266
AGACCGC169550.020.6068464
CCGCGTT172850.019.9398987
CGCGTTC174450.019.8209958
CAGACCG181050.019.4599233
ACGCGTC20850.017.70038840
CGTTACG34800.017.45600521
TCAGACC214300.017.102092
GCGTTCT210100.016.5434829
GCCGGCT198450.016.3920961
CCGGCTT234650.016.318842
CACGCGT24300.016.11442439
TTCACGC235750.015.6313247
CCGTACG43600.015.376864
CTCAGAC271150.015.3263871
CGCGTGA7350.015.3144141
AAGCGCG23700.015.18819518
GAACGAT228300.015.12045845
TTCGACG14700.014.9983096
Produced by FastQC (version 0.11.5)