FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45410147
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA505210.11125486997432535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG30400.013.46961326
GGAGGTC200100.013.3939924
GGGAGGT209300.013.0051253
ATAGGGG170350.012.22931838
GGCATAG170600.011.76306935
CGTAACG11250.011.39870942
CGTACGC7250.010.8629945
AGCTTAG189850.010.72419445
TAGACTC60550.010.6714943
TGGCATA191850.010.612782534
ATATAGC49500.010.4099096
GAGCTTA193500.010.26616744
TGTCGTT41000.010.26176928
GGTCACT258800.010.1725017
TAATCGG66150.010.1035024
TTAGACT65750.010.0681512
AATCGGT69400.010.0512895
GGTAACG62700.010.04671117
ATAATCG69500.010.0423273
GTAACGA64250.09.90947218
Produced by FastQC (version 0.11.5)