FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52636369
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC128250.019.9286234
GACCGCG131650.019.3797665
ACCGCGT140200.018.2460486
CAGACCG142050.018.1509653
CGTTACG24250.017.16432221
CCGCGTT152350.016.9532977
CGCGTTC155050.016.6725858
GAACGAT159850.016.1617845
CCCGGAT101750.014.35386851
CCCGTAT36250.014.2162851
CTCGCGT28050.014.1201221
GTCCGTT37650.014.1060581
AGAACGA185450.014.05211344
TCAGACC191100.014.0101842
GCGTTCT187000.013.9803879
CCGGCTT192350.013.7202942
ACGAACG23250.013.64492231
AATACGG188200.013.61692532
CTCCGAT55500.013.5023671
TACGGAG190950.013.32627534
Produced by FastQC (version 0.11.5)