FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45311881
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT875150.19313919013867467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT234200.018.67036445
TTCGACG16050.018.3583166
AGACCGC245400.018.0295564
CAGACCG250200.017.7286193
GACCGCG261100.016.8334435
ACCGCGT264550.016.6897226
CCGCGTT270300.016.3177577
CGCGTTC271700.016.258518
GCGTTCT294300.015.04039
TCAGACC309600.014.7849922
AGAACGA298350.014.67105644
CTCAGAC335000.014.6362451
GTTCCTA79900.014.41268515
CCCCCGT27800.014.0838611
ACGCGTC11400.014.03471640
CGTTACG17350.014.00059421
CCGTACG54850.013.9430434
GTCCGTT34400.013.9328021
CTCCGAT43200.013.7511011
CGTACGC56500.013.6951015
Produced by FastQC (version 0.11.5)