FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48319257
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG504740.10445938769298543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT276700.013.5151553
AGCTTAG185750.013.11835945
GGAGGTC281500.013.1008584
GGCATAG199050.012.15139435
ATAGGGG203450.012.12083938
GAGCTTA217250.011.34055144
TGGCATA221500.011.07216634
GTTGTCG19750.010.82272826
ACTCTCC344000.010.49775511
GCGTTCG19450.010.17988615
CATAGGG246150.010.11876737
TGGGAGG261400.010.0711582
GGTCACT367000.09.8092037
GCATAGG252800.09.80808736
CACTCTC376200.09.7965910
GTCACTC387550.09.4748488
GCTCTGT385550.09.37811322
CTGGGAG324000.09.3336921
CACCCGG93600.09.327281
TAGGCTA83850.09.31121310
Produced by FastQC (version 0.11.5)