FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51580645
Sequences flagged as poor quality0
Sequence length51-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG214400.031.7862595
ACCGCGT215550.031.6298586
AGACCGC228050.030.0738874
CCGCGTT236950.028.846127
CAGACCG239300.028.8297463
CCTACGC40600.028.7107864-65
CGCGTTC243300.028.0813878
CCCTACG43000.027.63469164-65
CTACGCC43200.027.50685766-67
ACACTAT195450.025.91180886-87
ACTATAG192150.025.88558888-89
TCAGACC294900.023.837182
CTAGTAA216550.023.83112778-79
GCGTTCT300300.022.8856779
CTAGGCG46400.022.4393982-83
TATAGAA207900.021.71990890-91
CTCAGAC363350.021.6913361
TAACACA242750.021.18926282-83
ACCCCTA46300.020.28772470-71
CACTATA208700.020.19962788-89
Produced by FastQC (version 0.11.5)