FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50993126
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1681390.3297287559895818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT306650.027.14327845
AGACCGC366150.022.9622784
AGAACGA367950.022.83522844
CAGACCG378500.022.4151973
ACCGCGT394450.021.289016
GACCGCG393450.021.2832165
CCGCGTT400700.020.9577357
CGCGTTC407150.020.5686198
AAGAACG410700.020.46925243
AATACGG423850.019.9513232
TCAGACC432300.019.8963132
ATACGGA426800.019.80855633
TACGGAG430900.019.63052234
GCGTTCT428550.019.567759
CAATACG438700.019.32726331
CTCAGAC471100.019.0362911
ACGGAGA453050.018.60123335
CGGAGAG464400.018.08846936
CCCAATA472650.017.89466329
ACTCCCC484700.017.5333825
Produced by FastQC (version 0.11.5)