FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45117298
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3905530.8656391612813338No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA720350.15966160030239399No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG702650.15573849302766313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT685500.027.93827445
AGACCGC780850.026.9191064
CAGACCG788800.026.6800613
GACCGCG793700.026.3954055
ACCGCGT807500.026.0050376
CCGCGTT817600.025.6695677
CGCGTTC819250.025.637098
TCAGACC831600.025.6234912
GCGTTCT843400.024.9270029
CTCAGAC890150.024.7587431
AGAACGA783500.024.41758344
CGTTCTC916850.023.19261710
ACTCCCC847750.022.84563625
CCTCTCA864000.022.6017419
TCACTCC879700.022.25615123
AAGAACG859950.022.21986643
CACTCCC886050.022.03825824
CTCACTC913350.021.46321522
AATACGG896200.021.35656732
ATACGGA896550.021.3244433
Produced by FastQC (version 0.11.5)