FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005202153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005202153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59312463
Sequences flagged as poor quality0
Sequence length51-101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT330200.016.5149483
GGAGGTC335750.015.9451164
CGCCGTA53750.013.92537694-95
GCGTCGG45350.013.86736694-95
GTCACTC378600.013.7554518
CGTTGAA181750.013.73054886-87
GTCGCCG87650.013.5135794-95
GGTCACT389450.013.3465877
GTAGGGA1104100.013.15300294-95
GAGGTCA418900.013.121995
TGTAGGG1208550.013.08889294-95
TTAGGGC188150.013.04473448-49
CACAACG239650.012.8102282-83
TAATCGG86750.012.6875074
TCGTGTA1542200.012.5762794-95
CTTAGGG199300.012.509315546-47
GTCGTGT1687250.012.30459294-95
GTGTAGG1380550.012.25465594-95
TAGGGAA1142300.012.24028994-95
AGCGTCG2196850.012.21990194-95
Produced by FastQC (version 0.11.5)